Author: Silva, E.R.M.; Watanabe, A.S.A.; Carraro, E.; Perosa, A.H.S.; Granato, C.F.H.; Bellei, N.C.J.
Title: Rhinovirus detection using different PCR-based strategies Document date: 2012_6_1
ID: sluqq1px_4
Snippet: In the present study, we aimed to compare three strategies for HRV detection in respiratory samples. The first strategy was a picornavirus RT-PCR hybridization assay, the second strategy was a rhinovirus RT-PCR sequencing approach and the third strategy was a semi-nested-RT-PCR for rhinovirus and enterovirus. The second protocol used RT-PCR with rhinovirusspecific primers that target a hypervariable fragment of the 5'UTR, the entire VP4 gene and .....
Document: In the present study, we aimed to compare three strategies for HRV detection in respiratory samples. The first strategy was a picornavirus RT-PCR hybridization assay, the second strategy was a rhinovirus RT-PCR sequencing approach and the third strategy was a semi-nested-RT-PCR for rhinovirus and enterovirus. The second protocol used RT-PCR with rhinovirusspecific primers that target a hypervariable fragment of the 5'UTR, the entire VP4 gene and the 5' terminus of the VP2 gene, followed by gene sequencing (20) .
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