Author: Robledo-Aceves, Mireya; Moreno-Peregrina, María de Jesús; Velarde-Rivera, Fernando; Ascencio-Esparza, Elba; Preciado-Figueroa, Francisco M.; Caniza, Miguela A.; Escobedo-Melendez, Griselda
Title: Risk factors for severe bronchiolitis caused by respiratory virus infections among Mexican children in an emergency department Document date: 2018_3_2
ID: uzuwqy3g_12
Snippet: Nasopharyngeal samples were obtained upon admission from all children with a diagnosis of severe bronchiolitis who were Robledo-Aceves et al. Medicine (2018) 97:9 Medicine enrolled in the study. The presence of respiratory virus was analyzed using a standard procedure. [31] Viruses were placed in viral transport medium and maintained at 4°C. The samples were stored at -80°C until their viral nucleic contents were analyzed. Respiratory viruses w.....
Document: Nasopharyngeal samples were obtained upon admission from all children with a diagnosis of severe bronchiolitis who were Robledo-Aceves et al. Medicine (2018) 97:9 Medicine enrolled in the study. The presence of respiratory virus was analyzed using a standard procedure. [31] Viruses were placed in viral transport medium and maintained at 4°C. The samples were stored at -80°C until their viral nucleic contents were analyzed. Respiratory viruses were detected and identified using the CLART PneumoVir array assay (Genomica S.A.U., Madrid, Spain) in 3 steps (extraction, amplification, and hybridization array), according to the manufacturer's protocol. This kit is based on 2 end-point multiplex reverse transcription-polymerase chain reaction (RT-PCR) or PCR amplification and subsequent DNA microarray (hybridization) detection. This assay detects single or mixed infections with 17 different respiratory viruses: adenovirus; bocavirus; coronavirus; enterovirus (echovirus); influenza A virus H1N1/2009; influenza viruses A, B, and C; metapneumoviruses A and B; parainfluenza viruses (PIV) type 1, 2, 3, 4A, and 4B; rhinoviruses; and RSV A and B. Sensitivity and specificity values of 90% or more are reported per the manufacturer.
Search related documents:
Co phrase search for related documents- array assay and chain reaction: 1, 2, 3, 4, 5, 6
- array assay and dna microarray: 1, 2
- array assay and hybridization detection: 1, 2
- array assay and influenza virus: 1, 2
- array assay and parainfluenza virus: 1
- array assay and PCR amplification: 1
- array assay and PneumoVir array assay: 1, 2
- array assay and respiratory virus: 1, 2, 3
- array assay and Spain Madrid Genomica PneumoVir array assay: 1
- array assay and transcription polymerase: 1, 2
- array assay and transcription polymerase chain reaction: 1
- array assay and virus influenza: 1, 2
- assay detect and chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72
- assay detect and different respiratory virus: 1, 2, 3
- assay detect and dna microarray: 1, 2, 3, 4, 5, 6, 7
- assay detect and end point: 1
- assay detect and hybridization detection: 1, 2, 3, 4
- assay detect and influenza virus: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50
- assay detect and influenza virus virus influenza: 1, 2, 3, 4
Co phrase search for related documents, hyperlinks ordered by date