Selected article for: "dual luciferase and luciferase reporter"
Author: Yu, Chien-Hung; Noteborn, Mathieu H.; Pleij, Cornelis W. A.; Olsthoorn, René C. L.
Title: Stem–loop structures can effectively substitute for an RNA pseudoknot in -1 ribosomal frameshifting
  • Document date: 2011_7_29
    • ID: wifs97yy_26
    Snippet: To further examine the role of the loop identity or size in ribosomal frameshifting, we cloned some of the above loop mutants into a dual-luciferase reporter plasmid and assayed their frameshifting efficiency in mammalian cells ( Figure 5B ). Our data show that the effects of loop nucleotides are comparable in vitro and in vivo. The stable GAAA tetraloop construct again had the lowest frameshifting efficiency ( Figure 5B , 2.9%), which was half t.....
    Document: To further examine the role of the loop identity or size in ribosomal frameshifting, we cloned some of the above loop mutants into a dual-luciferase reporter plasmid and assayed their frameshifting efficiency in mammalian cells ( Figure 5B ). Our data show that the effects of loop nucleotides are comparable in vitro and in vivo. The stable GAAA tetraloop construct again had the lowest frameshifting efficiency ( Figure 5B , 2.9%), which was half that of the UUCG construct ( Figure 5B, 6 .1%).

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