Author: Ishimaru, Daniella; Plant, Ewan P.; Sims, Amy C.; Yount, Boyd L.; Roth, Braden M.; Eldho, Nadukkudy V.; Pérez-Alvarado, Gabriela C.; Armbruster, David W.; Baric, Ralph S.; Dinman, Jonathan D.; Taylor, Deborah R.; Hennig, Mirko
Title: RNA dimerization plays a role in ribosomal frameshifting of the SARS coronavirus Document date: 2012_12_26
ID: zrbn637z_8
Snippet: In vitro transcriptions were optimized and performed as described (1, 18, 19) using unlabeled nucleotide triphosphates (MP Biomedicals). RNA transcripts were purified by fast-performance liquid chromatography with HiTrap Q column (GE Healthcare) (18, 19) . S3L2-only transcripts and variants were purified with a HiTrap Q (GE Healthcare), followed by a DNAPac PA200 columns (Dionex) (18) . Purified RNA was equilibrated with nuclear magnetic resonanc.....
Document: In vitro transcriptions were optimized and performed as described (1, 18, 19) using unlabeled nucleotide triphosphates (MP Biomedicals). RNA transcripts were purified by fast-performance liquid chromatography with HiTrap Q column (GE Healthcare) (18, 19) . S3L2-only transcripts and variants were purified with a HiTrap Q (GE Healthcare), followed by a DNAPac PA200 columns (Dionex) (18) . Purified RNA was equilibrated with nuclear magnetic resonance (NMR) buffer [25 mM KCl unless otherwise noted, 10 mM sodium phosphate (pH 6.5), 500 mM EDTA (Ethylenediaminetetraacetic acid), 50 mM sodium azide, 9:1 H 2 O:D 2 O].
Search related documents:
Co phrase search for related documents- fast performance and liquid chromatography: 1, 2, 3
- fast performance and magnetic resonance: 1
- liquid chromatography and magnetic resonance: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- liquid chromatography and NMR nuclear magnetic resonance: 1, 2, 3
- liquid chromatography and nuclear magnetic resonance: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- liquid chromatography and nucleotide triphosphate: 1, 2
- magnetic resonance and NMR nuclear magnetic resonance: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- magnetic resonance and nuclear magnetic resonance: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- magnetic resonance and sodium phosphate: 1
- mm sodium azide and sodium azide: 1, 2, 3, 4, 5, 6
- mm sodium azide and sodium phosphate: 1
- NMR nuclear magnetic resonance and nuclear magnetic resonance: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- nuclear magnetic resonance and sodium phosphate: 1
- pH 10 mm sodium phosphate and sodium phosphate: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- pH 10 mm sodium phosphate and vitro transcription: 1
- rna transcript and vitro transcription: 1
- sodium phosphate and vitro transcription: 1
Co phrase search for related documents, hyperlinks ordered by date