Author: Ishimaru, Daniella; Plant, Ewan P.; Sims, Amy C.; Yount, Boyd L.; Roth, Braden M.; Eldho, Nadukkudy V.; Pérez-Alvarado, Gabriela C.; Armbruster, David W.; Baric, Ralph S.; Dinman, Jonathan D.; Taylor, Deborah R.; Hennig, Mirko
Title: RNA dimerization plays a role in ribosomal frameshifting of the SARS coronavirus Document date: 2012_12_26
ID: zrbn637z_8
Snippet: In vitro transcriptions were optimized and performed as described (1, 18, 19) using unlabeled nucleotide triphosphates (MP Biomedicals). RNA transcripts were purified by fast-performance liquid chromatography with HiTrap Q column (GE Healthcare) (18, 19) . S3L2-only transcripts and variants were purified with a HiTrap Q (GE Healthcare), followed by a DNAPac PA200 columns (Dionex) (18) . Purified RNA was equilibrated with nuclear magnetic resonanc.....
Document: In vitro transcriptions were optimized and performed as described (1, 18, 19) using unlabeled nucleotide triphosphates (MP Biomedicals). RNA transcripts were purified by fast-performance liquid chromatography with HiTrap Q column (GE Healthcare) (18, 19) . S3L2-only transcripts and variants were purified with a HiTrap Q (GE Healthcare), followed by a DNAPac PA200 columns (Dionex) (18) . Purified RNA was equilibrated with nuclear magnetic resonance (NMR) buffer [25 mM KCl unless otherwise noted, 10 mM sodium phosphate (pH 6.5), 500 mM EDTA (Ethylenediaminetetraacetic acid), 50 mM sodium azide, 9:1 H 2 O:D 2 O].
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