Selected article for: "eosin staining and immunohistochemistry eosin staining"
Title: RESEARCH COMMUNICATIONS OF THE 28th ECVIM-CA CONGRESS
  • Document date: 2018_12_19
    • ID: r79h9yzz_9
    Snippet: This prospective study was conducted, with owner compliance, in the same referral centre between July 2016 and February 2018. Cats were included if they had a final diagnosis of LGAL or IBD. Haematoxylin and Eosin staining and immunohistochemistry using CD3, BLA36, MAC387 and MIB‐1 (Ki67) antibodies were carried out on full‐thickness intestinal biopsies. Markers expressions in lamina propria and intra‐epithelial lymphocytes (IEL) were asses.....
    Document: This prospective study was conducted, with owner compliance, in the same referral centre between July 2016 and February 2018. Cats were included if they had a final diagnosis of LGAL or IBD. Haematoxylin and Eosin staining and immunohistochemistry using CD3, BLA36, MAC387 and MIB‐1 (Ki67) antibodies were carried out on full‐thickness intestinal biopsies. Markers expressions in lamina propria and intra‐epithelial lymphocytes (IEL) were assessed separately and quantified using a semi‐quantitative scale. Blinded reading was performed by two board‐certified veterinary pathologists and one pathologist specialised in human digestive low‐grade proliferative disorders. All samples were subjected to clonality testing, based on PCR targeting of the CDR3 region of T‐cell receptor gamma for T‐cells (TCRγ).

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