Selected article for: "cdna synthesis and total rna"
Author: Wang, Jiying; Wang, Yanping; Wang, Huaizhong; Hao, Xiaojing; Wu, Ying; Guo, Jianfeng
Title: Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation
  • Document date: 2014_4_23
    • ID: xf4yy6i1_18
    Snippet: Total RNA of the samples of PBMC and whole blood was extracted using the TRIzol reagent, and their quality was measured by spectrophotometer. For the RNA of PBMC samples, the ratio A260/A280 was 1.940.25 (averageSD), and the concentration 78.2 ng/L48.5 (averageSD). For the RNA of whole blood samples, the ratio A260/A280 was 2.000.06 (averageSD), and the concentration 273.9 ng/L93.6 (averageSD). Our results indicated .....
    Document: Total RNA of the samples of PBMC and whole blood was extracted using the TRIzol reagent, and their quality was measured by spectrophotometer. For the RNA of PBMC samples, the ratio A260/A280 was 1.940.25 (averageSD), and the concentration 78.2 ng/L48.5 (averageSD). For the RNA of whole blood samples, the ratio A260/A280 was 2.000.06 (averageSD), and the concentration 273.9 ng/L93.6 (averageSD). Our results indicated that all the RNA samples were good in quality and suitable for the next analysis. However, the concentration of RNA from PMBC was quite low, and larger volume samples should be used in the next cDNA synthesis.

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