Selected article for: "gene expression and similar trend"
Author: Wang, Jiying; Wang, Yanping; Wang, Huaizhong; Hao, Xiaojing; Wu, Ying; Guo, Jianfeng
Title: Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation
  • Document date: 2014_4_23
    • ID: xf4yy6i1_28
    Snippet: The gene expression stability was also analyzed by another analysis program, NormFinder, where stable gene expression is indicated by lower average expression stability values. According to NormFinder results as shown in Figure 4 , the six candidate genes were more stably expressed in whole blood than PBMC, and also had a similar trend of expression stability in the sample of whole blood and PBMC. Especially, TBP was ranked as the most stable ref.....
    Document: The gene expression stability was also analyzed by another analysis program, NormFinder, where stable gene expression is indicated by lower average expression stability values. According to NormFinder results as shown in Figure 4 , the six candidate genes were more stably expressed in whole blood than PBMC, and also had a similar trend of expression stability in the sample of whole blood and PBMC. Especially, TBP was ranked as the most stable reference gene in both whole blood and PBMC, while B2M and PPIA were the second and third most stable ones. NormFinder found the stability value for best combination of two genes, TBP and PPIA, were 0.636 and 1.623 for whole blood and PBMC respectively. In accordance with results valuated by geNorm, the frequently used reference genes ACTB and GAPDH exhibited substantial variation, and were among the three least stable genes.

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