Title: Selective anchoring in the specific plasma membrane domain: a role in epithelial cell polarity Document date: 1988_12_1
ID: tyb0g7pz_27
Snippet: While only 6 % of AI was extracted from the apical sur-face, >70 % was extracted from the basal and lateral membrane domains (Table II, E) . Conversely, B1 and B2 showed small apical (incorrect domain) fluorescence peaks in unextracted cells (Fig. 4 , Cand E), which were decreased in size, although not completely abolished, by TX-100 (Fig. 4 , D and F). Levels of B1 and B2 extractability were •50% in the apical surface and '~16% in the lateral .....
Document: While only 6 % of AI was extracted from the apical sur-face, >70 % was extracted from the basal and lateral membrane domains (Table II, E) . Conversely, B1 and B2 showed small apical (incorrect domain) fluorescence peaks in unextracted cells (Fig. 4 , Cand E), which were decreased in size, although not completely abolished, by TX-100 (Fig. 4 , D and F). Levels of B1 and B2 extractability were •50% in the apical surface and '~16% in the lateral surface (Table II, E) . The other basolateral marker, B2-m, exhibited a similar pattern of extraction; like B2, it showed intermediate levels of extraction from the basal surface; like B1 and B2, the maximum insolubility in TX-100 was observed on the lateral surface.
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