Title: Retention of p63 in an ER-Golgi intermediate compartment depends on the presence of all three of its domains and on its ability to form oligomers Document date: 1994_7_1
ID: ra20actc_13
Snippet: The mutant forms of the p63 gene were created using standard PCR protocols (Ho et al., 1989) . All mutants start at bp78 of the original wild type (wt) p63 eDNA (Schweizer et al., 1993b) . The final PCR products were digested with BamHI and used to replace the BamHI-BamHI fragment of pBSK-p63. The entire PCR-derived fragment was sequenced by the dideoxy termination procedure (Sanger et al., 1977) as described (Schweizer et al., 1993b) . Correct c.....
Document: The mutant forms of the p63 gene were created using standard PCR protocols (Ho et al., 1989) . All mutants start at bp78 of the original wild type (wt) p63 eDNA (Schweizer et al., 1993b) . The final PCR products were digested with BamHI and used to replace the BamHI-BamHI fragment of pBSK-p63. The entire PCR-derived fragment was sequenced by the dideoxy termination procedure (Sanger et al., 1977) as described (Schweizer et al., 1993b) . Correct clones were subcloned into the EcoRI site of the pECE vector for transient expression in COS cells.
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