Author: Li, Ji Lian; Cornman, R. Scott; Evans, Jay D.; Pettis, Jeffery S.; Zhao, Yan; Murphy, Charles; Peng, Wen Jun; Wu, Jie; Hamilton, Michele; Boncristiani, Humberto F.; Zhou, Liang; Hammond, John; Chen, Yan Ping
Title: Systemic Spread and Propagation of a Plant-Pathogenic Virus in European Honeybees, Apis mellifera Document date: 2014_1_21
ID: wxiazglk_29
Snippet: Total RNA extraction and conventional RT-PCR. Total RNA was isolated from disserted tissues, adult bees, bee pollen, and Varroa mites using Invitrogen Trizol reagent according to the manufacturer's instructions. Conventional RT-PCR was performed on RNA samples extracted from adult bees, Varroa mites, different tissues, and bee bread collected from the same colony for the presence and distribution of TRSV. The Promega one-step Access RT-PCR system.....
Document: Total RNA extraction and conventional RT-PCR. Total RNA was isolated from disserted tissues, adult bees, bee pollen, and Varroa mites using Invitrogen Trizol reagent according to the manufacturer's instructions. Conventional RT-PCR was performed on RNA samples extracted from adult bees, Varroa mites, different tissues, and bee bread collected from the same colony for the presence and distribution of TRSV. The Promega one-step Access RT-PCR system was used for virus detection as previously described (58) . PCR products were purified and sequenced to confirm the specificity of the primers.
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