Selected article for: "genome sequencing and viral sequence"
Author: Peña, José; Chen-Harris, Haiyin; Allen, Jonathan E.; Hwang, Mona; Elsheikh, Maher; Mabery, Shalini; Bielefeldt-Ohmann, Helle; Zemla, Adam T.; Bowen, Richard A.; Borucki, Monica K.
Title: Sendai virus intra-host population dynamics and host immunocompetence influence viral virulence during in vivo passage
  • Document date: 2016_4_9
    • ID: z7f720dj_52
    Snippet: Illumina deep sequencing of the SeV genome was used to determine whether changes at the consensus or subconsensus level occurred. Sequence data was obtained for up to 99% of the SeV genome (nts 50-15315; numbering according to SeV Z strain (GI: 546225824)). Depth of coverage for most regions was between 10,000Â and 100,000Â. Prior to analyzing variants in the SeV samples, we verified that the UDS allowed detection of very rare variants. To cont.....
    Document: Illumina deep sequencing of the SeV genome was used to determine whether changes at the consensus or subconsensus level occurred. Sequence data was obtained for up to 99% of the SeV genome (nts 50-15315; numbering according to SeV Z strain (GI: 546225824)). Depth of coverage for most regions was between 10,000Â and 100,000Â. Prior to analyzing variants in the SeV samples, we verified that the UDS allowed detection of very rare variants. To control for PCR error, three control plasmids containing viral sequence insertions from SeV, RABV or BCoV were mixed at the ratio of 100,000:1,000:10 (SeV:RABV:BCoV), PCR amplified and sequenced.

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