Selected article for: "chain heavy chain and light chain"
Author: Rappuoli, Rino; Bottomley, Matthew J.; D’Oro, Ugo; Finco, Oretta; De Gregorio, Ennio
Title: Reverse vaccinology 2.0: Human immunology instructs vaccine antigen design
  • Document date: 2016_4_4
    • ID: uyoerxvu_29
    Snippet: More recently, an improved version of the multimeric germline-targeting antigen, eOD-GT8, has been used in two independent studies to immunize knock-in mice for the human VH1-2*02 variable heavy chain (Dosenovic et al., 2015; Jardine et al., 2015) . Both studies have shown that eOD-GT8 was able to expand B cells expressing human transgenic heavy chain combined to a mouse light chain with a five-amino acids-long CDR3 resembling naturally occurring.....
    Document: More recently, an improved version of the multimeric germline-targeting antigen, eOD-GT8, has been used in two independent studies to immunize knock-in mice for the human VH1-2*02 variable heavy chain (Dosenovic et al., 2015; Jardine et al., 2015) . Both studies have shown that eOD-GT8 was able to expand B cells expressing human transgenic heavy chain combined to a mouse light chain with a five-amino acids-long CDR3 resembling naturally occurring light chains of human VRC01, introducing a series of somatic mutations in the VH1-2*02 gene similar to those observed in naturally occurring HIV bNAbs. In contrast to eOD-GT8, native Env trimers were unable to expand VH1-2*02 B cells in the same knock-in mice. Importantly, in both studies, the Abs generated by immunization with eOD-GT8, although similar to VRC01, were not able to neutralize HIV infection.

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