Author: Enora Dupas; Bruno Legendre; Valérie Olivier; Françoise Poliakoff; Charles Manceau; Amandine Cunty
Title: Comparison of real-time PCR and droplet digital PCR for the detection of Xylella fastidiosa in plants Document date: 2019_3_20
ID: 0xc02kkb_20
Snippet: In this work, we proposed the first suitable ddPCR assay for the detection of Xf in plants. We 389 easily transferred a well-known routinely used real-time PCR technique for Xf detection in 390 ddPCR. Here, we reported all the set up steps leading to the optimal protocol and its comparison 391 with the current routine method. The results demonstrated the usefulness of ddPCR technology 392 as an alternative method for Xf detection in plants. Howev.....
Document: In this work, we proposed the first suitable ddPCR assay for the detection of Xf in plants. We 389 easily transferred a well-known routinely used real-time PCR technique for Xf detection in 390 ddPCR. Here, we reported all the set up steps leading to the optimal protocol and its comparison 391 with the current routine method. The results demonstrated the usefulness of ddPCR technology 392 as an alternative method for Xf detection in plants. However, the ddPCR assay is more time-393 consuming than real-time PCR and does not seem to be suitable for routine analysis. This 394 technology requires more steps than real-time PCR. Furthermore, the reaction mix has to handle 395 with care to ensure the generation of the appropriate number of generated droplets. 396
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