Author: Ma, Ge; Greenwell-Wild, Teresa; Lei, Kejian; Jin, Wenwen; Swisher, Jennifer; Hardegen, Neil; Wild, Carl T.; Wahl, Sharon M.
Title: Secretory Leukocyte Protease Inhibitor Binds to Annexin II, a Cofactor for Macrophage HIV-1 Infection Document date: 2004_11_15
ID: rlabxfss_14
Snippet: Cell-free Binding Assay. 96-well plates (Nunc immunoplates with maxiSorp surface; Nunc) were coated with 60 g/ml BSA (Sigma-Aldrich), annexin II tetramer, IgG, or gp120 (NIH AIDS Research and Reference Reagent Program) overnight at 4 Њ C, washed with PBS, and blocked or not with 1% BSA for 1-2 h. After two PBS washes, 100 l HIV-1 BaL (10 4 /ml) was added for 1 h at 4 Њ C (3). After multiple washes with PBS, 100 l of lysis buffer (0.5% Triton X-.....
Document: Cell-free Binding Assay. 96-well plates (Nunc immunoplates with maxiSorp surface; Nunc) were coated with 60 g/ml BSA (Sigma-Aldrich), annexin II tetramer, IgG, or gp120 (NIH AIDS Research and Reference Reagent Program) overnight at 4 Њ C, washed with PBS, and blocked or not with 1% BSA for 1-2 h. After two PBS washes, 100 l HIV-1 BaL (10 4 /ml) was added for 1 h at 4 Њ C (3). After multiple washes with PBS, 100 l of lysis buffer (0.5% Triton X-100) was added. Virus was quantified by p24 ELISA.
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