Author: Liu, Yuan-yuan; Chen, Liang-jun; Zhong, Yan; Shen, Meng-xin; Ma, Nian; Liu, Bing-yu; Luo, Fan; Hou, Wei; Yang, Zhan-qiu; Xiong, Hai-rong
Title: Specific interference shRNA-expressing plasmids inhibit Hantaan virus infection in vitro and in vivo Document date: 2016_3_14
ID: pwlybr2h_14
Snippet: One day before transfection, the Vero-E6 cells were seeded into 24-well plates at 5×10 4 cells per well. The 80%-90% confluent cells were then transfected with 0.25, 0.5, 1, 2, or 4 μg of the pSilencer-S, pSilencer-M or pSilencer 3.0 H1 (as pSilencerblank) vectors with Lipofectamine 2000 (Invitrogen) according to the manufacturer's instruction. The transfection efficiencies were according to GFP expression of the co-transfected plasmid pEGFP-C1.....
Document: One day before transfection, the Vero-E6 cells were seeded into 24-well plates at 5×10 4 cells per well. The 80%-90% confluent cells were then transfected with 0.25, 0.5, 1, 2, or 4 μg of the pSilencer-S, pSilencer-M or pSilencer 3.0 H1 (as pSilencerblank) vectors with Lipofectamine 2000 (Invitrogen) according to the manufacturer's instruction. The transfection efficiencies were according to GFP expression of the co-transfected plasmid pEGFP-C1. After 24 hpi, the cells were infected with 100 TCID 50 /0.2 mL of HTNV76-118. At 1, 2, 3, 5, 7, and 9 day post-infection (dpi), antigen slides were prepared to detect the HTNV protein expressions by IFA.
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