Author: Liu, Yuan-yuan; Chen, Liang-jun; Zhong, Yan; Shen, Meng-xin; Ma, Nian; Liu, Bing-yu; Luo, Fan; Hou, Wei; Yang, Zhan-qiu; Xiong, Hai-rong
Title: Specific interference shRNA-expressing plasmids inhibit Hantaan virus infection in vitro and in vivo Document date: 2016_3_14
ID: pwlybr2h_18
Snippet: Specific pathogen-free pregnant BALB/c mice were obtained from the Animal Research Center of Wuhan University. Suckling mice (2 d old) were intracranially inoculated with 20 μL HTNV 76-118 viral stock at 100 LD 50 . The mice were randomly divided into 4 groups (30 mice per group) and intraperitoneally injected with 10 μL of the plasmids (pSilencer-S, pSilencer-M or pSilencer 3.0 H1 vectors at concentrations of 3 μg/μL) or 10 μL PBS for the n.....
Document: Specific pathogen-free pregnant BALB/c mice were obtained from the Animal Research Center of Wuhan University. Suckling mice (2 d old) were intracranially inoculated with 20 μL HTNV 76-118 viral stock at 100 LD 50 . The mice were randomly divided into 4 groups (30 mice per group) and intraperitoneally injected with 10 μL of the plasmids (pSilencer-S, pSilencer-M or pSilencer 3.0 H1 vectors at concentrations of 3 μg/μL) or 10 μL PBS for the normal controls and viral controls at 1 and 3 dpi, respectively. The treatments were performed for the survival rate study (n=10); these mice were observed daily for 15 d following infection, and the mortality rate and mean time to death (MTD) were estimated.
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