Author: Liu, Yuan-yuan; Chen, Liang-jun; Zhong, Yan; Shen, Meng-xin; Ma, Nian; Liu, Bing-yu; Luo, Fan; Hou, Wei; Yang, Zhan-qiu; Xiong, Hai-rong
Title: Specific interference shRNA-expressing plasmids inhibit Hantaan virus infection in vitro and in vivo Document date: 2016_3_14
ID: pwlybr2h_8
Snippet: The DNA template was synthesized by Sangon Biotech (Shanghai) Co, Ltd, and the shRNA was generated using the MessageMuterâ„¢ shRNA production kit (Qiagen) as described previously [12] . The Vero-E6 cells were grown in 24-well plates to 80%-90% confluence and transfected with specific or control shRNA (60 nmol/L) with RNAiFect transfection reagent (Qiagen, Germantown, MD, USA). At 24 h after transfection, the cells were infected with 100 TCID 50 H.....
Document: The DNA template was synthesized by Sangon Biotech (Shanghai) Co, Ltd, and the shRNA was generated using the MessageMuter™ shRNA production kit (Qiagen) as described previously [12] . The Vero-E6 cells were grown in 24-well plates to 80%-90% confluence and transfected with specific or control shRNA (60 nmol/L) with RNAiFect transfection reagent (Qiagen, Germantown, MD, USA). At 24 h after transfection, the cells were infected with 100 TCID 50 HTNV 76-118 (100 μL per well), and viral RNA was detected by qRT-PCR at 24 or 48 hour post-infection (hpi) to determine the interference efficiency. The viral titers were determined at 96 hpi. The shRNA S1 and shRNA M2 were found to be the most effective shRNAs in terms of the inhibition of HTNV and were selected for use in the subsequent experiments.
Search related documents:
Co phrase search for related documents, hyperlinks ordered by date