Selected article for: "expression stability and reference gene"
Author: Wang, Jiying; Wang, Yanping; Wang, Huaizhong; Hao, Xiaojing; Wu, Ying; Guo, Jianfeng
Title: Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation
  • Document date: 2014_4_23
    • ID: xf4yy6i1_25
    Snippet: Assuming that the reference genes are not co-regulated, stepwise exclusion of the gene with the highest M value results in a combination of two constitutively expressed reference genes that have the most stable expression in the tested samples (Vandesompele et al., 2002) . Figure 2a and 2b shows the average expression stability values of remaining control genes for whole blood and PBMC. Combining using the two reference genes for normalization, B.....
    Document: Assuming that the reference genes are not co-regulated, stepwise exclusion of the gene with the highest M value results in a combination of two constitutively expressed reference genes that have the most stable expression in the tested samples (Vandesompele et al., 2002) . Figure 2a and 2b shows the average expression stability values of remaining control genes for whole blood and PBMC. Combining using the two reference genes for normalization, B2M and PPIA, the average expression stability values can obtain rather small M values for the samples of whole blood (0.299) and PBMC (0.490).

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