Author: Wang, Jiying; Wang, Yanping; Wang, Huaizhong; Hao, Xiaojing; Wu, Ying; Guo, Jianfeng
Title: Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation Document date: 2014_4_23
ID: xf4yy6i1_6
Snippet: PBMC were isolated using Ficoll-Hypaque PLUS (GE healthcare) following the manufacturer's instructions. In brief, the whole blood was firstly diluted by an equal volume of balanced salt solution (PBS). Then, 15 mL of diluted blood was carefully added on the top of 10 mL of Ficoll-Hypaque solution in a 50 mL conical tube and centrifuged at 460ï‚´g for 20 min at room temperature. After centrifugation, the middle whitish interface containing mononuc.....
Document: PBMC were isolated using Ficoll-Hypaque PLUS (GE healthcare) following the manufacturer's instructions. In brief, the whole blood was firstly diluted by an equal volume of balanced salt solution (PBS). Then, 15 mL of diluted blood was carefully added on the top of 10 mL of Ficoll-Hypaque solution in a 50 mL conical tube and centrifuged at 460ï‚´g for 20 min at room temperature. After centrifugation, the middle whitish interface containing mononuclear cells was transferred to a new tube, and washed by adding 25 mL PBS followed by centrifugation at 150ï‚´g for 10 min two times.
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