Author: Peña, José; Chen-Harris, Haiyin; Allen, Jonathan E.; Hwang, Mona; Elsheikh, Maher; Mabery, Shalini; Bielefeldt-Ohmann, Helle; Zemla, Adam T.; Bowen, Richard A.; Borucki, Monica K.
Title: Sendai virus intra-host population dynamics and host immunocompetence influence viral virulence during in vivo passage Document date: 2016_4_9
ID: z7f720dj_27
Snippet: Software was created to link Illumina reads aligned to regions nearby on the reference and construct viral haplotypes. The resulting haplotypes were experimentally verified using cloning and Sanger sequencing. The haplotype building software linked non-overlapping read pairs from Illumina data and thus allowed linkage of mutations that are separated by as much as the length of both read pairs (300 bp). Fisher's exact test was used to determine st.....
Document: Software was created to link Illumina reads aligned to regions nearby on the reference and construct viral haplotypes. The resulting haplotypes were experimentally verified using cloning and Sanger sequencing. The haplotype building software linked non-overlapping read pairs from Illumina data and thus allowed linkage of mutations that are separated by as much as the length of both read pairs (300 bp). Fisher's exact test was used to determine statistical significance for each linkage.
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