Selected article for: "situ hybridization and tight junction protein"
Title: RESEARCH COMMUNICATIONS OF THE 28th ECVIM-CA CONGRESS
  • Document date: 2018_12_19
    • ID: r79h9yzz_57
    Snippet: Eight dogs diagnosed with moderate‐to‐severe IBD (CIBDAI score > 6) were treated with immunosuppressive doses of glucocorticoids, (oral prednisone 1‐2 mg/kg PO every 24 hours for 21 days, then tapered gradually to 0.5 mg/kg at 8 weeks as a typical protocol), for induction of clinical remission. The mucosal microbiota from endoscopic intestinal biopsies of IBD dogs and controls (n=15 dogs) was evaluated by fluorescence in situ hybridization .....
    Document: Eight dogs diagnosed with moderate‐to‐severe IBD (CIBDAI score > 6) were treated with immunosuppressive doses of glucocorticoids, (oral prednisone 1‐2 mg/kg PO every 24 hours for 21 days, then tapered gradually to 0.5 mg/kg at 8 weeks as a typical protocol), for induction of clinical remission. The mucosal microbiota from endoscopic intestinal biopsies of IBD dogs and controls (n=15 dogs) was evaluated by fluorescence in situ hybridization (FISH) targeting the 16S rRNA genes of total bacteria, group‐specific organisms, and individual bacterial species shown to be relevant in canine/human IBD. Epithelial tight junction protein (TJP) expression was studied using immunohistochemistry to investigate the effect of drug therapy on intestinal barrier integrity.

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