Author: Shefali Dobhal; Gamze Boluk; Brooke Babler; Michael J. Stulberg; John Rascoe; Mark Nakhla; Toni A. Chapman; Alex B. Crockford; Michael Melzer; Anne M. Alvarez; Mohammad Arif
Title: Comparative genomics reveals signature regions used to develop a robust and sensitive multiplex TaqMan real-time qPCR assay to detect the genus Dickeya and Dickeya dianthicola Document date: 2019_11_20
ID: lgeu4id0_35
Snippet: In plant disease management, rapid, sensitive and accurate detection of pathogens is critical for timely responses that facilitate disease control. Furthermore, early disease detection is important for assessing plant health prior to seedling transplant. In this study, we developed a robust multiplex TaqMan real-time qPCR for simultaneous detection of all known Dickeya species and D. dianthicola in a single reaction. The primers were developed fr.....
Document: In plant disease management, rapid, sensitive and accurate detection of pathogens is critical for timely responses that facilitate disease control. Furthermore, early disease detection is important for assessing plant health prior to seedling transplant. In this study, we developed a robust multiplex TaqMan real-time qPCR for simultaneous detection of all known Dickeya species and D. dianthicola in a single reaction. The primers were developed from unique and conserved genes, extracted using comparative genomic analyses of Dickeya species. The developed method has been thoroughly validated using strains in inclusivity and exclusivity panels as well as naturally and artificially infected samples. The method showed 100% accuracy and successfully detected infection of both Dickeya species and D. dianthicola from infected plant samples received from different states of the United States.
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