Author: Blank, Maximilian F.; Chen, Sifan; Poetz, Fabian; Schnölzer, Martina; Voit, Renate; Grummt, Ingrid
Title: SIRT7-dependent deacetylation of CDK9 activates RNA polymerase II transcription Document date: 2017_3_17
ID: qm9urt2w_10
Snippet: The mgf-files generated by Xcalibur software (Thermo Scientific) were used for database searches with the MAS-COT search engine (Matrix Science, version 2.4) against the SwissProt database (SwissProt version 2014 04). The peptide mass tolerance was set to 5 ppm and fragment mass tolerance to 0.4 Da. Proteins were considered as identified if more than one unique peptide had an individual ion score exceeding the MASCOT identity threshold. GO analys.....
Document: The mgf-files generated by Xcalibur software (Thermo Scientific) were used for database searches with the MAS-COT search engine (Matrix Science, version 2.4) against the SwissProt database (SwissProt version 2014 04). The peptide mass tolerance was set to 5 ppm and fragment mass tolerance to 0.4 Da. Proteins were considered as identified if more than one unique peptide had an individual ion score exceeding the MASCOT identity threshold. GO analysis was performed using DAVID Bioinformatics Resources (19, 20) . Assessment of changes in the proteome was based on the relation of unique peptides in the treated sample and the control sample after substraction of IgG values, a ratio below 0.72 was considered as decreased interaction.
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