Author: Blank, Maximilian F.; Chen, Sifan; Poetz, Fabian; Schnölzer, Martina; Voit, Renate; Grummt, Ingrid
Title: SIRT7-dependent deacetylation of CDK9 activates RNA polymerase II transcription Document date: 2017_3_17
ID: qm9urt2w_36
Snippet: In a complementary approach, we monitored binding of radiolabeled RNA to immobilized wildtype and mutant SIRT7. Again, deletion of the N-terminal 78 amino acids markedly reduced RNA binding ( Figure 2B and Supplementary Figure S2C ). In accord with previous RNA immunoprecipitation (CLIP) experiments showing that SIRT7 not only interacts with pre-rRNA but also with snoRNAs (13), deletion of the N-terminal part of SIRT7 impaired the interaction of .....
Document: In a complementary approach, we monitored binding of radiolabeled RNA to immobilized wildtype and mutant SIRT7. Again, deletion of the N-terminal 78 amino acids markedly reduced RNA binding ( Figure 2B and Supplementary Figure S2C ). In accord with previous RNA immunoprecipitation (CLIP) experiments showing that SIRT7 not only interacts with pre-rRNA but also with snoRNAs (13), deletion of the N-terminal part of SIRT7 impaired the interaction of SIRT7 with pre-rRNA, U3, U13, U14 and 88c snoRNAs ( Figure 2C ). Moreover, SIRT7/ N78 was distributed throughout the nucleus, indicating that the N-terminal region mediates nucleolar enrichment of SIRT7 ( Figure 2D ).
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