Author: Stewart, Meredith E.; Roy, Polly
Title: Structure-based identification of functional residues in the nucleoside-2'-O-methylase domain of Bluetongue virus VP4 capping enzyme Document date: 2015_2_24
ID: vzel6r43_21
Snippet: To ensure that the mutations did not interfere with the structure of the mutant proteins, the stable interaction with VP4 capping enzyme and GMP moiety was undertaken according to Le Blois et al. [14] with the following modification: 2 lg of purified recombinant protein and 0.5 lCi/ll of a 32 P-GTP were used......
Document: To ensure that the mutations did not interfere with the structure of the mutant proteins, the stable interaction with VP4 capping enzyme and GMP moiety was undertaken according to Le Blois et al. [14] with the following modification: 2 lg of purified recombinant protein and 0.5 lCi/ll of a 32 P-GTP were used.
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