Selected article for: "copy number and gene copy number"
Author: Qin, Jian; Jones, Robert C.; Ramakrishnan, Ramesh
Title: Studying copy number variations using a nanofluidic platform
  • Document date: 2008_8_18
    • ID: prsvv6l9_35
    Snippet: Quantitative PCR is simple and easy to perform. However, since the copy number of the target gene is derived from the Ct difference between the target gene and a reference gene, the results are very sensitive to the efficiency of the amplification reaction. Even if one compensates for the amplification efficiency, it is considered difficult to obtain a discrimination power of better than twofold (66) ......
    Document: Quantitative PCR is simple and easy to perform. However, since the copy number of the target gene is derived from the Ct difference between the target gene and a reference gene, the results are very sensitive to the efficiency of the amplification reaction. Even if one compensates for the amplification efficiency, it is considered difficult to obtain a discrimination power of better than twofold (66) .

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