Author: Liu, Yuan-yuan; Chen, Liang-jun; Zhong, Yan; Shen, Meng-xin; Ma, Nian; Liu, Bing-yu; Luo, Fan; Hou, Wei; Yang, Zhan-qiu; Xiong, Hai-rong
Title: Specific interference shRNA-expressing plasmids inhibit Hantaan virus infection in vitro and in vivo Document date: 2016_3_14
ID: pwlybr2h_15
Snippet: In other parallel experiments, the cells from individual wells (2-μg plasmid transfection groups) were collected at 2 dpi for viral RNA detection. At 5 dpi, the cultures were subjected to two cycles of freezing and thawing followed by centrifugation at low speed (1000×g), and the supernatants were then serially diluted, inoculated on the cells, and finally titrated by IFA......
Document: In other parallel experiments, the cells from individual wells (2-μg plasmid transfection groups) were collected at 2 dpi for viral RNA detection. At 5 dpi, the cultures were subjected to two cycles of freezing and thawing followed by centrifugation at low speed (1000×g), and the supernatants were then serially diluted, inoculated on the cells, and finally titrated by IFA.
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