Selected article for: "dmem medium and mL penicillin"
Author: Liu, Yuan-yuan; Chen, Liang-jun; Zhong, Yan; Shen, Meng-xin; Ma, Nian; Liu, Bing-yu; Luo, Fan; Hou, Wei; Yang, Zhan-qiu; Xiong, Hai-rong
Title: Specific interference shRNA-expressing plasmids inhibit Hantaan virus infection in vitro and in vivo
  • Document date: 2016_3_14
    • ID: pwlybr2h_6
    Snippet: Vero-E6 cells were obtained from the China Center for Type Culture Collection (CCTCC) and maintained in DMEM supplemented with 10% fetal bovine serum (FBS, GIBCO, Carlsbad, CA, USA), 0.1% L-glutamine, 100 U/mL penicillin and streptomycin. DMEM containing 2% FBS was used to maintain the medium after viral infection. The stocks of Hantaan virus strain 76-118 were obtained from the Institute of Virology of the Chinese Academy of Preventive Medicine .....
    Document: Vero-E6 cells were obtained from the China Center for Type Culture Collection (CCTCC) and maintained in DMEM supplemented with 10% fetal bovine serum (FBS, GIBCO, Carlsbad, CA, USA), 0.1% L-glutamine, 100 U/mL penicillin and streptomycin. DMEM containing 2% FBS was used to maintain the medium after viral infection. The stocks of Hantaan virus strain 76-118 were obtained from the Institute of Virology of the Chinese Academy of Preventive Medicine (Beijing, China) and were propagated in Vero-E6 cells. HTNV titration was performed regularly based on indirect immunofluorescence assay (IFA) as described previously [18] . The TCID 50 was determined to be 10 6 /0.1 mL.

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