Author: Ma, Ge; Greenwell-Wild, Teresa; Lei, Kejian; Jin, Wenwen; Swisher, Jennifer; Hardegen, Neil; Wild, Carl T.; Wahl, Sharon M.
Title: Secretory Leukocyte Protease Inhibitor Binds to Annexin II, a Cofactor for Macrophage HIV-1 Infection Document date: 2004_11_15
ID: rlabxfss_17
Snippet: In previous studies (3), we documented that 125 I-rhSLPI binds to human monocytederived macrophages in a specific, concentration-dependent, and saturable manner. In the current studies, SLPI binding was detected on SLPI-treated human monocytes and macrophages with anti-SLPI antibody or using 1 g/ ml of a biotinylated rhSLPI and streptavidin-FITC with enhanced sensitivity. Direct binding of SLPI to macrophage membranes was detected in Õ† 85% of ma.....
Document: In previous studies (3), we documented that 125 I-rhSLPI binds to human monocytederived macrophages in a specific, concentration-dependent, and saturable manner. In the current studies, SLPI binding was detected on SLPI-treated human monocytes and macrophages with anti-SLPI antibody or using 1 g/ ml of a biotinylated rhSLPI and streptavidin-FITC with enhanced sensitivity. Direct binding of SLPI to macrophage membranes was detected in Õ† 85% of macrophages by flow cytometry (Fig. 1 A) . By fluorescence microscopy, cell surface rhSLPI-biotin-neutravidin-FITC complexes were evident within 1 min, peaked at 15-30 min (Fig. 1 B) , and frequently appeared to polarize before emerging in the cytosol, all consistent with a cell membrane-binding molecule/receptor.
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