Selected article for: "expression stability and stable reference gene"
Author: Wang, Jiying; Wang, Yanping; Wang, Huaizhong; Hao, Xiaojing; Wu, Ying; Guo, Jianfeng
Title: Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation
  • Document date: 2014_4_23
    • ID: xf4yy6i1_24
    Snippet: Gene expression stability measure M was calculated for the samples of whole blood and PBMC with or without poly I:C stimulation. Genes with the lowest M value have the most stable expression, while the highest M value has the least stable expression. The expression M values of the six reference genes are plotted in Figure 1 . It can be seen that, except more stably expressed in samples of whole blood than those of PBMC, the six candidate referenc.....
    Document: Gene expression stability measure M was calculated for the samples of whole blood and PBMC with or without poly I:C stimulation. Genes with the lowest M value have the most stable expression, while the highest M value has the least stable expression. The expression M values of the six reference genes are plotted in Figure 1 . It can be seen that, except more stably expressed in samples of whole blood than those of PBMC, the six candidate reference genes perform similar trend of expression stability in the sample of whole blood and PBMC. That is, B2M is the most stable reference gene with the lowest M value (0.631 and 0.777 in whole blood and PBMC, respectively), while ACTB the least stable one with the highest M value (0.921 and 1.426 in whole blood and PBMC, respectively).

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