Author: Wang, Jiying; Wang, Yanping; Wang, Huaizhong; Hao, Xiaojing; Wu, Ying; Guo, Jianfeng
Title: Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation Document date: 2014_4_23
ID: xf4yy6i1_8
Snippet: In order to assure a sufficient amount of cells for the next RNA extraction, samples of the three piglets with the same concentration of poly I:C and stimulation time were merged together for RNA extraction. Thus, we had 17 samples of PBMC, including five samples without poly I:C stimulation (one uncultured control and 4 samples collected after 3 h, 6 h, 12 h, and 24 h of culture) and 12 samples with poly I:C stimulation (4 time points, and each .....
Document: In order to assure a sufficient amount of cells for the next RNA extraction, samples of the three piglets with the same concentration of poly I:C and stimulation time were merged together for RNA extraction. Thus, we had 17 samples of PBMC, including five samples without poly I:C stimulation (one uncultured control and 4 samples collected after 3 h, 6 h, 12 h, and 24 h of culture) and 12 samples with poly I:C stimulation (4 time points, and each point with 3 different poly I:C concentration tests).
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