Title: Selective anchoring in the specific plasma membrane domain: a role in epithelial cell polarity Document date: 1988_12_1
ID: tyb0g7pz_28
Snippet: Since the A1 mAb binding site might involve a carbohydrate epitope (Table I) it was conceivable that the extractable fraction of A1 was composed of glycolipids carrying the same epitope as the 215-kD antigen. This possibility was ruled out by the following experiment: MDCK cell monolayers were grown on glass coverslips for 1 d and fixed in either 2% PFA at 4°C or 96% methanol at -20°C. Some coverslips were further extracted in chloroform/methan.....
Document: Since the A1 mAb binding site might involve a carbohydrate epitope (Table I) it was conceivable that the extractable fraction of A1 was composed of glycolipids carrying the same epitope as the 215-kD antigen. This possibility was ruled out by the following experiment: MDCK cell monolayers were grown on glass coverslips for 1 d and fixed in either 2% PFA at 4°C or 96% methanol at -20°C. Some coverslips were further extracted in chloroform/methanol 2:1. The latter extraction did not result in significant decrease of A1 binding in any case as determined by indirect RIA.
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