Selected article for: "ls reagent and ma ipswich new england biolabs"
Author: Peña, José; Chen-Harris, Haiyin; Allen, Jonathan E.; Hwang, Mona; Elsheikh, Maher; Mabery, Shalini; Bielefeldt-Ohmann, Helle; Zemla, Adam T.; Bowen, Richard A.; Borucki, Monica K.
Title: Sendai virus intra-host population dynamics and host immunocompetence influence viral virulence during in vivo passage
  • Document date: 2016_4_9
    • ID: z7f720dj_20
    Snippet: Total RNA from BAL or single plaque isolated virus supernatant was extracted using TRIzol LS Reagent (Invitrogen) following the manufacturer's protocol. Reverse transcription was performed using random hexamers and the Superscript III RT reverse transcriptase kit (Invitrogen). Viral cDNA templates were amplified using the Phusion polymerase kit (New England BioLabs, Ipswich, MA), following manufacturer's instructions. PCR conditions consisted of .....
    Document: Total RNA from BAL or single plaque isolated virus supernatant was extracted using TRIzol LS Reagent (Invitrogen) following the manufacturer's protocol. Reverse transcription was performed using random hexamers and the Superscript III RT reverse transcriptase kit (Invitrogen). Viral cDNA templates were amplified using the Phusion polymerase kit (New England BioLabs, Ipswich, MA), following manufacturer's instructions. PCR conditions consisted of 98 C for 30 s, followed by 40 cycles of 98 C for 15 s, 64 C for 20 s, and 72 C for 1.2 min. The final cycle was 72 C for 10 min. PCR products were prepared for sequencing using the QIAquick PCR Purification kit (Qiagen, Valencia, CA).

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