Selected article for: "respiratory tract cell and tract cell"
Author: Shen, Ching-I; Wang, Ching-Ho; Liao, Jiunn-Wang; Hsu, Tien-Wang; Kuo, Shu-Ming; Su, Hong-Lin
Title: The infection of primary avian tracheal epithelial cells with infectious bronchitis virus
  • Document date: 2009_10_1
    • ID: qs82fva6_25
    Snippet: It has been reported that IBV can infect both ciliated cells and goblet cells in the tracheal epithelium [1, 22] . However, whether basal cells are also susceptible to IBV infection remains obscure. We infected isolated intact tracheas with 50 lL 2575/98 (EID 50 = 10 5 /mL) for 48 h and found that IBV protein expression only colocalized with b-tubulin IV-or mucin 5AC-positive cells (Figs. 4A and 4B) . No viral protein was detected in the K14-posi.....
    Document: It has been reported that IBV can infect both ciliated cells and goblet cells in the tracheal epithelium [1, 22] . However, whether basal cells are also susceptible to IBV infection remains obscure. We infected isolated intact tracheas with 50 lL 2575/98 (EID 50 = 10 5 /mL) for 48 h and found that IBV protein expression only colocalized with b-tubulin IV-or mucin 5AC-positive cells (Figs. 4A and 4B) . No viral protein was detected in the K14-positive basal cells of the tracheal epithelium (Figs. 4C and 4D). To further examine whether basal cells are resistant to IBV infection in vitro, 5 · 10 4 ATE cells were also infected with 50 lL of IBV 2575/98 strain (10 5 EID 50 /mL) for 48 h. In vitro results consistently showed that viral proteins of IBV were mainly detected in ciliated cells and goblet cells, but not in basal cells (Figs. 4E-4H). Similar cell tropism results were obtained when IBV 2296/95 or a higher dosage of viral loading was used (data not shown). These in vivo and in vitro experiments clearly delineate the cell tropism of IBV in the avian respiratory tract.

    Search related documents:
    Co phrase search for related documents
    • ATE cell and cell tropism: 1
    • ATE cell and goblet cell: 1, 2
    • ATE cell and IBV cell tropism: 1
    • ATE cell and IBV infection: 1, 2
    • ATE cell and protein expression: 1
    • ATE cell and respiratory tract: 1
    • ATE cell and tracheal epithelium: 1, 2
    • avian respiratory tract and IBV infection: 1
    • avian respiratory tract and respiratory tract: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
    • basal cell and cell tropism: 1, 2, 3, 4, 5
    • basal cell and ciliated cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    • basal cell and goblet cell: 1, 2, 3, 4, 5, 6, 7
    • basal cell and protein expression: 1, 2
    • basal cell and respiratory tract: 1, 2, 3, 4, 5, 6, 7
    • basal cell and tracheal epithelium: 1
    • basal cell and viral protein: 1, 2
    • cell tropism and ciliated cell: 1, 2, 3, 4, 5, 6, 7, 8
    • cell tropism and goblet cell: 1, 2, 3
    • cell tropism and goblet cell ciliated cell: 1