Author: Zheng, Li-Zhen; Wang, Jia-Li; Kong, Ling; Huang, Le; Tian, Li; Pang, Qian-Qian; Wang, Xin-Luan; Qin, Ling
Title: Steroid-associated osteonecrosis animal model in rats Document date: 2018_2_6
ID: tad9jmfp_17
Snippet: Before euthanasia, blood was collected via cardiac puncture for serum isolation that was then stored at À80 C before the biomarkers assay. Bone formation marker, including amino-terminal propeptide of type I collagen (PINP) and osteocalcin (OC), and bone resorption marker, carboxy-terminal telopeptide (CTX), were assayed using enzyme-linked immunosorbent assay (ELISA) kits (IDS Ltd., Boldon, UK) [23] ......
Document: Before euthanasia, blood was collected via cardiac puncture for serum isolation that was then stored at À80 C before the biomarkers assay. Bone formation marker, including amino-terminal propeptide of type I collagen (PINP) and osteocalcin (OC), and bone resorption marker, carboxy-terminal telopeptide (CTX), were assayed using enzyme-linked immunosorbent assay (ELISA) kits (IDS Ltd., Boldon, UK) [23] .
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