Author: Silva, E.R.M.; Watanabe, A.S.A.; Carraro, E.; Perosa, A.H.S.; Granato, C.F.H.; Bellei, N.C.J.
Title: Rhinovirus detection using different PCR-based strategies Document date: 2012_6_1
ID: sluqq1px_13
Snippet: Finally, some authors have suggested that inclusion of capsid coding sequences (21) in the PCR target region may slightly decrease an assay's screening sensitivity compared with targeting the 5'UTR alone (10) However, authors using a capsid coding sequence protocol have pointed out that this strategy is more robust for genetic typing and has been used in other studies (5, 9, 11, 12, 18, 20, 21, 24) . In the present study the sequencing protocol, .....
Document: Finally, some authors have suggested that inclusion of capsid coding sequences (21) in the PCR target region may slightly decrease an assay's screening sensitivity compared with targeting the 5'UTR alone (10) However, authors using a capsid coding sequence protocol have pointed out that this strategy is more robust for genetic typing and has been used in other studies (5, 9, 11, 12, 18, 20, 21, 24) . In the present study the sequencing protocol, including capsid sequences, confirmed the previous data and was specific for detecting and confirming HRV-positive samples.
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