Selected article for: "cdna Synthesis kit and Fermentas cdna Synthesis kit"
Author: Li, Li; Wang, Li; Xiao, Ruijing; Zhu, Guoguo; Li, Yan; Liu, Changxuan; Yang, Ru; Tang, Zhiqing; Li, Jie; Huang, Wei; Chen, Lang; Zheng, Xiaoling; He, Yuling; Tan, Jinquan
Title: The invasion of tobacco mosaic virus RNA induces endoplasmic reticulum stress-related autophagy in HeLa cells
  • Document date: 2011_11_21
    • ID: r4c1sngt_17
    Snippet: Total RNA was extracted from HeLa cell using TRIzol ® (Invitrogen), according to the manufacturer's instructions. The synthesis of first cDNA strand was carried out as described by the manufacturer (RevertAid TM First strand cDNA synthesis kit; Fermentas). For real-time PCR, the sequence data were designed and obtained from GenBank ® . The Beclin1 sequence was 5 -ATACCGACTTGTTCCTTAC-3 (sense) and 5 -GTCTTCAATCTTGCCTTT-3 (antisense) and the β-a.....
    Document: Total RNA was extracted from HeLa cell using TRIzol ® (Invitrogen), according to the manufacturer's instructions. The synthesis of first cDNA strand was carried out as described by the manufacturer (RevertAid TM First strand cDNA synthesis kit; Fermentas). For real-time PCR, the sequence data were designed and obtained from GenBank ® . The Beclin1 sequence was 5 -ATACCGACTTGTTCCTTAC-3 (sense) and 5 -GTCTTCAATCTTGCCTTT-3 (antisense) and the β-actin sequence was 5 -TCACCCACACTGTGCCCCATCTACGA-3 (sense) and 5 -CAGCGGAACCGCTCATTGCCAATGG-3 (antisense).

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