Author: Stewart, Meredith E.; Roy, Polly
Title: Structure-based identification of functional residues in the nucleoside-2'-O-methylase domain of Bluetongue virus VP4 capping enzyme Document date: 2015_2_24
ID: vzel6r43_19
Snippet: A Renilla luciferase assay that detects the expression from BTV S10 (S10-Rluc) RNA reporter plasmid during virus infection was used to quantify the viral protein synthesis as established [2] . In brief, BSR monolayer cells in 96 well plates were transfected with S10-Rluc RNA followed by infection with either BTV or NYR mutant virus at 12 h post-transfection. Renilla luciferase activity was quantified at 12 h post infection using the Renilla Lucif.....
Document: A Renilla luciferase assay that detects the expression from BTV S10 (S10-Rluc) RNA reporter plasmid during virus infection was used to quantify the viral protein synthesis as established [2] . In brief, BSR monolayer cells in 96 well plates were transfected with S10-Rluc RNA followed by infection with either BTV or NYR mutant virus at 12 h post-transfection. Renilla luciferase activity was quantified at 12 h post infection using the Renilla Luciferase Assay System (Promega), according to the manufacturer's instructions. Each reaction was performed in triplicate and student t-test was used to determine significance.
Search related documents:
Co phrase search for related documents- luciferase activity and NYR mutant virus: 1
- luciferase activity and post infection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- luciferase activity and post transfection: 1, 2, 3, 4, 5, 6, 7, 8, 9
- luciferase activity and Promega Renilla Luciferase Assay System: 1, 2, 3, 4
- luciferase activity and protein synthesis: 1, 2, 3, 4, 5
- luciferase activity and Renilla luciferase: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- luciferase activity and Renilla luciferase activity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- luciferase activity and RNA reporter: 1, 2, 3, 4, 5, 6, 7
- mutant virus and NYR mutant virus: 1, 2
- mutant virus and post infection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- mutant virus and post transfection: 1
- mutant virus and protein synthesis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
- mutant virus and Renilla luciferase: 1, 2
- mutant virus and Renilla luciferase activity: 1
- NYR mutant virus and protein synthesis: 1
- NYR mutant virus and Renilla luciferase: 1
- NYR mutant virus and Renilla luciferase activity: 1
- post infection and protein synthesis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23
- post infection and Renilla luciferase: 1, 2, 3
Co phrase search for related documents, hyperlinks ordered by date