Author: Stewart, Meredith E.; Roy, Polly
Title: Structure-based identification of functional residues in the nucleoside-2'-O-methylase domain of Bluetongue virus VP4 capping enzyme Document date: 2015_2_24
ID: vzel6r43_19
Snippet: A Renilla luciferase assay that detects the expression from BTV S10 (S10-Rluc) RNA reporter plasmid during virus infection was used to quantify the viral protein synthesis as established [2] . In brief, BSR monolayer cells in 96 well plates were transfected with S10-Rluc RNA followed by infection with either BTV or NYR mutant virus at 12 h post-transfection. Renilla luciferase activity was quantified at 12 h post infection using the Renilla Lucif.....
Document: A Renilla luciferase assay that detects the expression from BTV S10 (S10-Rluc) RNA reporter plasmid during virus infection was used to quantify the viral protein synthesis as established [2] . In brief, BSR monolayer cells in 96 well plates were transfected with S10-Rluc RNA followed by infection with either BTV or NYR mutant virus at 12 h post-transfection. Renilla luciferase activity was quantified at 12 h post infection using the Renilla Luciferase Assay System (Promega), according to the manufacturer's instructions. Each reaction was performed in triplicate and student t-test was used to determine significance.
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