Author: Gendron, Karine; Ferbeyre, Gerardo; Heveker, Nikolaus; Brakier-Gingras, Léa
Title: The activity of the HIV-1 IRES is stimulated by oxidative stress and controlled by a negative regulatory element Document date: 2010_10_8
ID: qtn3ukf4_29
Snippet: Our aim was to obtain insights into the regulation of the activity of the IRES located in the 5 0 UTR of HIV-1 full-length mRNA through a mutational analysis. In this study, we describe RNA regions important for the activity of the HIV-1 5 0 UTR IRES. We found three positive determinants (nt 202-217, 240-242 and 302-305) whose presence increases the IRES activity $2-fold and one negative determinant, a stem-loop encompassing nt 134-178 called IRE.....
Document: Our aim was to obtain insights into the regulation of the activity of the IRES located in the 5 0 UTR of HIV-1 full-length mRNA through a mutational analysis. In this study, we describe RNA regions important for the activity of the HIV-1 5 0 UTR IRES. We found three positive determinants (nt 202-217, 240-242 and 302-305) whose presence increases the IRES activity $2-fold and one negative determinant, a stem-loop encompassing nt 134-178 called IRENE, which decreases the IRES activity $2-fold. We also found that oxidative stress increases the wild-type IRES activity $2-fold but not that of the M4.stem143 mutant IRES with a mutated IRENE, whose activity is $2-fold that of the wildtype. This is the first study showing that oxidative stress activates the 5 0 UTR IRES of HIV-1.
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