Selected article for: "negative determinant and wild type"
Author: Gendron, Karine; Ferbeyre, Gerardo; Heveker, Nikolaus; Brakier-Gingras, Léa
Title: The activity of the HIV-1 IRES is stimulated by oxidative stress and controlled by a negative regulatory element
  • Document date: 2010_10_8
    • ID: qtn3ukf4_29
    Snippet: Our aim was to obtain insights into the regulation of the activity of the IRES located in the 5 0 UTR of HIV-1 full-length mRNA through a mutational analysis. In this study, we describe RNA regions important for the activity of the HIV-1 5 0 UTR IRES. We found three positive determinants (nt 202-217, 240-242 and 302-305) whose presence increases the IRES activity $2-fold and one negative determinant, a stem-loop encompassing nt 134-178 called IRE.....
    Document: Our aim was to obtain insights into the regulation of the activity of the IRES located in the 5 0 UTR of HIV-1 full-length mRNA through a mutational analysis. In this study, we describe RNA regions important for the activity of the HIV-1 5 0 UTR IRES. We found three positive determinants (nt 202-217, 240-242 and 302-305) whose presence increases the IRES activity $2-fold and one negative determinant, a stem-loop encompassing nt 134-178 called IRENE, which decreases the IRES activity $2-fold. We also found that oxidative stress increases the wild-type IRES activity $2-fold but not that of the M4.stem143 mutant IRES with a mutated IRENE, whose activity is $2-fold that of the wildtype. This is the first study showing that oxidative stress activates the 5 0 UTR IRES of HIV-1.

    Search related documents:
    Co phrase search for related documents
    • IRES activity increase and oxidative stress: 1, 2, 3, 4, 5
    • IRES activity increase and wild type: 1, 2, 3, 4
    • IRES activity increase and wild type IRES activity: 1, 2, 3, 4
    • IRES activity increase and wild type IRES activity increase: 1
    • mutational analysis and negative determinant: 1
    • mutational analysis and oxidative stress: 1, 2
    • mutational analysis and RNA region: 1, 2, 3, 4, 5
    • mutational analysis and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23
    • mutational analysis and wild type IRES activity: 1, 2
    • mutational analysis and wild type IRES activity increase: 1
    • negative determinant and positive determinant: 1, 2
    • negative determinant and wild type: 1, 2
    • negative determinant and wild type IRES activity: 1
    • oxidative stress and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34
    • oxidative stress and wild type IRES activity: 1, 2, 3, 4, 5, 6, 7
    • oxidative stress and wild type IRES activity increase: 1
    • positive determinant and wild type: 1
    • positive determinant and wild type IRES activity: 1
    • RNA region and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23