Selected article for: "equal protein amount and protein amount"
Author: Kenworthy, Rachael; Lambert, Diana; Yang, Feng; Wang, Nan; Chen, Zihong; Zhu, Haizhen; Zhu, Fanxiu; Liu, Chen; Li, Kui; Tang, Hengli
Title: Short-hairpin RNAs delivered by lentiviral vector transduction trigger RIG-I-mediated IFN activation
  • Document date: 2009_9_3
    • ID: uvf5qzfd_8
    Snippet: Protein contents of cell lysate were quantified with the Bio-Rad DC protein assay (Bio-Rad, Hercules, CA, USA), and an equal amount of total protein was loaded in each lane. Samples for IRF-3 dimerization assay were run on a polyacrylamide gel under non-denaturing conditions (35) . Other samples were denatured and separated by sodium dodecyl sulfate polyacrylamide gelelectrophoresis (SDS-PAGE). Proteins were then transferred onto a nitrocellulose.....
    Document: Protein contents of cell lysate were quantified with the Bio-Rad DC protein assay (Bio-Rad, Hercules, CA, USA), and an equal amount of total protein was loaded in each lane. Samples for IRF-3 dimerization assay were run on a polyacrylamide gel under non-denaturing conditions (35) . Other samples were denatured and separated by sodium dodecyl sulfate polyacrylamide gelelectrophoresis (SDS-PAGE). Proteins were then transferred onto a nitrocellulose membrane and stained with the appropriate antibodies with the SNAP i.d. TM system (Millipore, Worcester, MA, USA) according to the manufacturer's instructions.

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