Author: Blank, Maximilian F.; Chen, Sifan; Poetz, Fabian; Schnölzer, Martina; Voit, Renate; Grummt, Ingrid
Title: SIRT7-dependent deacetylation of CDK9 activates RNA polymerase II transcription Document date: 2017_3_17
ID: qm9urt2w_25
Snippet: Immunopurified Flag-RPB1 or GST-CTD (Sigma) was incubated for 1 h at 30 • C in kinase buffer (150 mM KCl, 50 mM Hepes pH [8.0], 10 mM MgCl 2 , 6 mM EDTA, 1 mM DTT, 50 M ATP, 5 Ci ⥠[ 32 P]-ATP) with bead-bound HA-CDK9. Phosphorylation was visualized by Phospho-rImaging or on western blots with anti-phospho-Ser2 antibodies......
Document: Immunopurified Flag-RPB1 or GST-CTD (Sigma) was incubated for 1 h at 30 • C in kinase buffer (150 mM KCl, 50 mM Hepes pH [8.0], 10 mM MgCl 2 , 6 mM EDTA, 1 mM DTT, 50 M ATP, 5 Ci ⥠[ 32 P]-ATP) with bead-bound HA-CDK9. Phosphorylation was visualized by Phospho-rImaging or on western blots with anti-phospho-Ser2 antibodies.
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