Author: Hu, Yongwu; Wen, Jie; Tang, Lin; Zhang, Haijun; Zhang, Xiaowei; Li, Yan; Wang, Jing; Han, Yujun; Li, Guoqing; Shi, Jianping; Tian, Xiangjun; Jiang, Feng; Zhao, Xiaoqian; Wang, Jun; Liu, Siqi; Zeng, Changqing; Wang, Jian; Yang, Huanming
Title: The M Protein of SARS-CoV: Basic Structural and Immunological Properties Document date: 2016_11_28
ID: xzlcyn3l_41
Snippet: Samples from the SARS patients, their processing and preparation, as well as sequencing have been described previously (6). Briefly, 6 isolates of coronavirus-like virus were isolated from Vero-E6 cell cultures infected with tissues from SARS patients. Virus preparation, RT-PCR and cloning were carried out according to standard protocols at BGI (Beijing Genomics Institute, http://www.genomics.org.cn). Sequencing was performed with MegaBACE 1000 (.....
Document: Samples from the SARS patients, their processing and preparation, as well as sequencing have been described previously (6). Briefly, 6 isolates of coronavirus-like virus were isolated from Vero-E6 cell cultures infected with tissues from SARS patients. Virus preparation, RT-PCR and cloning were carried out according to standard protocols at BGI (Beijing Genomics Institute, http://www.genomics.org.cn). Sequencing was performed with MegaBACE 1000 (Amersham, New Jersey, USA).
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