Selected article for: "frameshift efficiency and slippery sequence"
Author: Liao, Pei-Yu; Choi, Yong Seok; Dinman, Jonathan D.; Lee, Kelvin H.
Title: The many paths to frameshifting: kinetic modelling and analysis of the effects of different elongation steps on programmed –1 ribosomal frameshifting
  • Document date: 2010_9_7
    • ID: wwq0sd2r_35
    Snippet: To further our understanding of two types of frameshift proteins, the HTLV-1 pro-pol frameshift sequence was cloned into the reporter system. The extended frameshift sequence for HTLV-1 pro-pol is C CCU UUA AAC (where spaces separate zero frame codons and the slippery sequence is underlined). Similar to MB2CCC, the E-site tRNA GGG Pro can potentially form three canonical base pairings with the À1 frame CCC, which may create a favorable condition.....
    Document: To further our understanding of two types of frameshift proteins, the HTLV-1 pro-pol frameshift sequence was cloned into the reporter system. The extended frameshift sequence for HTLV-1 pro-pol is C CCU UUA AAC (where spaces separate zero frame codons and the slippery sequence is underlined). Similar to MB2CCC, the E-site tRNA GGG Pro can potentially form three canonical base pairings with the À1 frame CCC, which may create a favorable condition for Pathway I. Consequently, a significant amount of FS m among total frameshift proteins can be produced. Consistent with the model, the frameshift efficiency for HTLV-1 was 4.81% and the fraction of FS m was 39.4%.

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