Author: Tavakoli, Ahmad; Karbalaie Niya, Mohammad Hadi; Bokharaei-Salim, Farah; Farahmand, Mohammad; Izadi, Morteza; Dorostkar, Ruhollah; Keyvani, Hossein
Title: The molecular epidemiology of respiratory viruses in military trainees in Iran Document date: 2019_5_8
ID: txsjrcs3_10
Snippet: This system was based on reverse transcription polymerase chain reaction (RT-PCR) for amplifying a specific 120–330 bp target of the viral genome. RT-PCR was performed in two different ready-to-use amplification tubes. Amplified products were labeled with biotin in the course of the process. After the amplification, biotin-labeled products were hybridized with their respective specific probes immobilized at different sites in the array. Next, t.....
Document: This system was based on reverse transcription polymerase chain reaction (RT-PCR) for amplifying a specific 120–330 bp target of the viral genome. RT-PCR was performed in two different ready-to-use amplification tubes. Amplified products were labeled with biotin in the course of the process. After the amplification, biotin-labeled products were hybridized with their respective specific probes immobilized at different sites in the array. Next, the samples were incubated with the streptavidin-peroxidase to generate insoluble products which precipitate at the hybridization sites in the array. Hybridization happened in a low-density microarray containing triplicate probes specific to viruses.
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