Selected article for: "dimer formation and loop loop"
Author: Ishimaru, Daniella; Plant, Ewan P.; Sims, Amy C.; Yount, Boyd L.; Roth, Braden M.; Eldho, Nadukkudy V.; Pérez-Alvarado, Gabriela C.; Armbruster, David W.; Baric, Ralph S.; Dinman, Jonathan D.; Taylor, Deborah R.; Hennig, Mirko
Title: RNA dimerization plays a role in ribosomal frameshifting of the SARS coronavirus
  • Document date: 2012_12_26
    • ID: zrbn637z_59
    Snippet: When we capped Stem 3 with a stable CUUG tetraloop that normally facilitates formation of stable hairpin structures (54) and is highly conserved (55) , the frequency of frameshifting almost returned to wild-type levels ( Figure 8) . Surprisingly, in the context of the SARS-CoV Stem 3 sequence, 5 0 -cuug-3 0 tetraloop-capped mutants readily formed extended duplex structures as revealed by native gel and NMR analysis. Thus, the promotion of wild-ty.....
    Document: When we capped Stem 3 with a stable CUUG tetraloop that normally facilitates formation of stable hairpin structures (54) and is highly conserved (55) , the frequency of frameshifting almost returned to wild-type levels ( Figure 8) . Surprisingly, in the context of the SARS-CoV Stem 3 sequence, 5 0 -cuug-3 0 tetraloop-capped mutants readily formed extended duplex structures as revealed by native gel and NMR analysis. Thus, the promotion of wild-type levels of frameshifting exhibited by S3-cuug and S3-2bp-cuug SARS pseudoknot variants is consistent with a mechanism involving dimer formation. The metastable SARS-CoV Stem 3 sequence, which features a G38 · U59 wobble pair and a bulged A56, apparently evolved to facilitate a certain degree of dimerization if seeded through loop-loop kissing interactions that can even tolerate tandem U-U mismatches.

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