Selected article for: "genomic rna and primer set"
Author: Li, Ji Lian; Cornman, R. Scott; Evans, Jay D.; Pettis, Jeffery S.; Zhao, Yan; Murphy, Charles; Peng, Wen Jun; Wu, Jie; Hamilton, Michele; Boncristiani, Humberto F.; Zhou, Liang; Hammond, John; Chen, Yan Ping
Title: Systemic Spread and Propagation of a Plant-Pathogenic Virus in European Honeybees, Apis mellifera
  • Document date: 2014_1_21
    • ID: wxiazglk_33
    Snippet: In situ hybridization. Purified amplicons corresponding to the region flanked by the TRSV-F2 and TRSV-R2 primer set were incorporated into a pCR2.1 TA cloning vector upstream of a T7 promoter (Invitrogen, Carlsbad, CA) following the manufacturer's protocol. Recombinant plasmid DNAs with the TRSV insert were linearized by restriction enzyme BamHI (New England Biolabs, Ipswich, MA) at 37°C for 2 h. The linearized DNAs were extracted once with an e.....
    Document: In situ hybridization. Purified amplicons corresponding to the region flanked by the TRSV-F2 and TRSV-R2 primer set were incorporated into a pCR2.1 TA cloning vector upstream of a T7 promoter (Invitrogen, Carlsbad, CA) following the manufacturer's protocol. Recombinant plasmid DNAs with the TRSV insert were linearized by restriction enzyme BamHI (New England Biolabs, Ipswich, MA) at 37°C for 2 h. The linearized DNAs were extracted once with an equal volume of phenolchloroform-isoamyl alcohol (25:24:1), precipitated by ethanol, and dissolved in nuclease water. The DIG-labeled RNA probe complementary to TRSV genomic RNA was synthesized using a DIG-RNA labeling kit (T7) (Roche Applied Science, Indianapolis, IN) following the manufacturer's protocol.

    Search related documents:
    Co phrase search for related documents
    • equal volume and primer set: 1
    • linearized dna and nuclease water: 1
    • linearized dna and plasmid dna: 1, 2, 3, 4, 5, 6
    • linearized dna and restriction enzyme: 1, 2
    • manufacturer protocol and plasmid dna: 1, 2
    • nuclease water and plasmid dna: 1, 2, 3
    • nuclease water and primer set: 1, 2, 3, 4, 5
    • plasmid dna and primer set: 1, 2
    • plasmid dna and restriction enzyme: 1, 2, 3, 4, 5, 6, 7, 8
    • plasmid dna and RNA probe: 1
    • primer set and restriction enzyme: 1, 2
    • primer set and RNA probe: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10