Author: Galindo, I; Hernáez, B; Muñoz-Moreno, R; Cuesta-Geijo, M A; Dalmau-Mena, I; Alonso, C
Title: The ATF6 branch of unfolded protein response and apoptosis are activated to promote African swine fever virus infection Document date: 2012_7_5
ID: qfm61wmx_20
Snippet: Antibodies used for western blotting included the following: rabbit polyclonal antibody to calreticulin (ab2907 abcam, Cambridge, UK) at a dilution of 1 : 3000; rabbit polyclonal antibody to calnexin (StressGen, San Diego, CA, USA) 1 : 3000; rabbit polyclonal to Bap31 (ab37120 abcam) 1 : 3000; rabbit polyclonal antibody to ERp57 (ab10287 abcam) 1 : 1000; goat polyclonal to GADD34 (ab9869 abcam) 1 : 1000; rabbit polyclonal antibody GADD153/CHOP (F.....
Document: Antibodies used for western blotting included the following: rabbit polyclonal antibody to calreticulin (ab2907 abcam, Cambridge, UK) at a dilution of 1 : 3000; rabbit polyclonal antibody to calnexin (StressGen, San Diego, CA, USA) 1 : 3000; rabbit polyclonal to Bap31 (ab37120 abcam) 1 : 3000; rabbit polyclonal antibody to ERp57 (ab10287 abcam) 1 : 1000; goat polyclonal to GADD34 (ab9869 abcam) 1 : 1000; rabbit polyclonal antibody GADD153/CHOP (F-168) (Santa Cruz Biotechnology, Santa Cruz, CA, USA) 1 : 200; rabbit polyclonal antibody to GRP78 (BiP) (Santa Cruz Biotechnology) 1 : 200; rabbit polyclonal antibody to ATF4 (ab85049) 1 : 500 mouse monoclonal antibody to PDI (ab2792 abcam) 1 : 1000; antib-actin (Sigma, St Louis, MI, USA) 1 : 1500; anti-ASFV p30 monoclonal antibody 1 : 1000; and anti-p72 monoclonal antibody clone 18BG3 (Ingenasa, Madrid, Spain) 1 : 5000. The following secondary antibodies were used: goat anti-rabbit IgG HPR conjugate (Bio-Rad) and sheep anti-mouse IgG HPR conjugate (GE Healthcare), observing the manufacturer's instructions. Band densitometry was performed with TINA image analysis software and data were normalized to control values.
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